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Class 12 Bio

1. Plant Anatomy Syllabus

Unit 1: Plant Anatomy Notes

1. Plant Anatomy
1.1.1 Concept of tissue
1.1.2 Types of plant tissues (meristems and permanent tissues)
1.1.3 Anatomy of dicot and monocot root
1.1.4 Anatomy of dicot and monocot stem
1.1.5 Anatomy of dicot and monocot leaf
1.1.6 Secondary growth of dicot stem

These plant anatomy notes follow the NEB Class 12 Biology syllabus (+2 Biology, Nepal). Practise the multiple choice questions first, then work through the solved short and long answer questions with their diagrams, and finish with the PDF notes for quick revision.

Micro syllabus: 1. Plant Anatomy

Explain the concept of tissues and classify plant tissues.
Describe the anatomical structure of root, stem, and leaf of monocot and dicot plants.
Define and explain the mechanism of secondary growth in dicot stems.

Read more about plant anatomy, the meristem and secondary growth on Wikipedia. More notes: Class 12 Biology notes, Class 12 Chemistry notes and Class 12 Physics notes.

2. Plant Anatomy Notes: Multiple Choice Questions

Question 1. Plant anatomy deals with

  • a) Heredity and variations
  • b) Metabolic activities in plants
  • c) Structure and function of plant tissues
  • d) Reproduction in plants

Correct Answer: c) Structure and function of plant tissues

Reason: Plant anatomy is the study of the internal structure of plant parts, mainly their tissues. Metabolism belongs to plant physiology.


Question 2. Who is known as the ‘father of plant anatomy’?

  • a) N. Grew
  • b) G. Mendel
  • c) R. Hooke
  • d) Theophrastus

Correct Answer: a) N. Grew

Reason: Nehemiah Grew (1641-1712) made the first detailed microscopic study of plant parts and coined the terms tissue and parenchyma.


Question 3. Which tissue makes the outer lining of plant parts?

  • a) Cambium
  • b) Epidermis
  • c) Pith
  • d) Phloem

Correct Answer: b) Epidermis

Reason: Epidermis is the outermost protective layer of the young plant body. Cambium, pith and phloem lie inside.


Question 4. Meristematic cell has

  • a) Large vacuole
  • b) No nucleus
  • c) No cell wall
  • d) High rate of cell division

Correct Answer: d) High rate of cell division

Reason: Meristematic cells are young and keep dividing by mitosis. They have a big nucleus and dense cytoplasm, and vacuoles are absent or very small.


Question 5. Which is the correct pair?

  • a) Intercalary meristem: root length
  • b) Lateral meristem: plant height
  • c) Apical meristem: inter-nodal length
  • d) Root apex: root length

Correct Answer: d) Root apex: root length

Reason: The apical meristem at the root apex increases the length of the root. Lateral meristem increases girth, and intercalary meristem increases inter-nodal length.


Question 6. Lateral meristem increases

  • a) Height of the plant
  • b) Inter-nodal length of the plant
  • c) Cross-section area of the plant
  • d) Number of flowers and fruits in plants

Correct Answer: c) Cross-section area of the plant

Reason: Lateral meristem (vascular cambium and cork cambium) divides sideways and adds thickness (girth) to stem and root.


Question 7. Parenchyma that stores cell inclusions is

  • a) Aerenchyma
  • b) Prosenchyma
  • c) Chlorenchyma
  • d) Idioblast

Correct Answer: d) Idioblast

Reason: Idioblasts are large parenchyma cells that store inclusions such as tannins, oils and crystals.


Question 8. Cell wall of collenchyma consists of

  • a) Cellulose
  • b) Cellulose and pectin
  • c) Cellulose and lignin
  • d) Lignin

Correct Answer: b) Cellulose and pectin

Reason: Collenchyma walls are unevenly thickened by pectin and cellulose. Lignin is found in sclerenchyma.


Question 9. Aerenchyma is commonly present in

  • a) Xerophytes
  • b) Stem of woody trees
  • c) Hydrophytes
  • d) Alpine plants

Correct Answer: c) Hydrophytes

Reason: Aerenchyma has air cavities that make aquatic plants light and buoyant.


Question 10. An elongated, narrow, non-living cell with tapering ends is

  • a) Fibre
  • b) Sclereid
  • c) Guard cell
  • d) Bulliform cell

Correct Answer: a) Fibre

Reason: A fibre is a long, narrow, spindle-shaped dead cell with pointed ends. A sclereid is short and isodiametric.


Question 11. Living mechanical tissue is

  • a) Parenchyma
  • b) Collenchyma
  • c) Sclerenchyma
  • d) Idioblast

Correct Answer: b) Collenchyma

Reason: Collenchyma supports the plant while its cells stay alive. Sclerenchyma is dead.


Question 12. The empty space inside the sclerenchyma fibre is called

  • a) Pit
  • b) Plasmodesma
  • c) Lumen
  • d) Lignification

Correct Answer: c) Lumen

Reason: Heavy lignin thickening leaves only a narrow central cavity, called the lumen.

Question 13. Which of the following is a heterogeneous tissue?

  • a) Meristem
  • b) Parenchyma
  • c) Sclerenchyma
  • d) Xylem

Correct Answer: d) Xylem

Reason: Xylem is a complex tissue made of tracheids, vessels, xylem parenchyma and xylem fibres. The others have one kind of cell.


Question 14. Plant tissue which transports prepared food from leaf to other parts is

  • a) Phloem
  • b) Xylem
  • c) Cambium
  • d) Sclerenchyma

Correct Answer: a) Phloem

Reason: Phloem carries organic food from the leaf to storage organs and all other parts. Xylem carries water and minerals.


Question 15. Which of the following is not a component of xylem?

  • a) Vessel
  • b) Tracheid
  • c) Sieve tube
  • d) Wood fibre

Correct Answer: c) Sieve tube

Reason: Sieve tubes belong to phloem.


Question 16. Phloem consists of all living cells except

  • a) Phloem parenchyma
  • b) Phloem fibre
  • c) Companion cell
  • d) Sieve tube

Correct Answer: b) Phloem fibre

Reason: Phloem fibres are thick-walled, lignified and dead. The other three are living.


Question 17. In root of angiosperms, vascular bundles are

  • a) Radial type
  • b) Concentric type
  • c) Conjoint and collateral type
  • d) Conjoint and bicollateral type

Correct Answer: a) Radial type

Reason: In roots, xylem and phloem lie on different radii, alternately. This is the radial arrangement.


Question 18. When xylem is surrounded by phloem outside, the vascular bundle is said to be

  • a) Concentric
  • b) Amphicribral
  • c) Amphivasal
  • d) Radial

Correct Answer: b) Amphicribral

Reason: Xylem in the centre and phloem all around it is the amphicribral type. In amphivasal, phloem is in the centre. (Concentric is the general group, so the exact type is amphicribral.)


Question 19. In exarch xylem, protoxylem lies

  • a) Towards the pith, inner to metaxylem
  • b) Towards the periphery, outer to metaxylem
  • c) In between outer and inner metaxylems
  • d) At centre, and gets completely surrounded by metaxylem

Correct Answer: b) Towards the periphery, outer to metaxylem

Reason: Exarch condition is seen in roots: protoxylem is near the pericycle and metaxylem is towards the centre.


Question 20. Which of the following ceases conducting function of xylem elements?

  • a) Callose
  • b) Tylose
  • c) Suberin
  • d) Lignin

Correct Answer: b) Tylose

Reason: Tyloses are outgrowths of parenchyma that fill the lumen of vessels and block water flow in old wood (heartwood).


Question 21. Which is the best pair?

  • a) Periblem = periderm + phellem + phellogen
  • b) Periderm = phellem + phellogen + phelloderm
  • c) Phelloderm = periderm + phellem + phelloderm
  • d) Periblem = plerome + phellem + phellogen

Correct Answer: b) Periderm = phellem + phellogen + phelloderm

Reason: Periderm is the cork, the cork cambium and the secondary cortex together. Periblem is an apical histogen that forms the cortex.


Question 22. In leaf of a plant, which of the following is true about xylem?

  • a) Xylem is exarch
  • b) Xylem is endarch
  • c) It may be either exarch or endarch
  • d) Its arrangement cannot be defined

Correct Answer: b) Xylem is endarch

Reason: In a leaf the protoxylem lies towards the upper epidermis (inner side of the bundle) and metaxylem towards the phloem. So the xylem is endarch, as in the stem.


Question 23. With secondary growth over years, what changes might have seen in the trunk of a tree?

  • a) Heartwood expands, sapwood shrinks
  • b) Both heartwood and sapwood expand
  • c) Heartwood shrinks, sapwood expands
  • d) Both heartwood and sapwood shrink

Correct Answer: a) Heartwood expands, sapwood shrinks

Reason: Old inner sapwood turns into heartwood by tylosis and deposits, so heartwood keeps increasing while the living sapwood stays a thin outer layer.

3. Plant Anatomy Notes: Short and Long Answer Questions

Short Answer Questions

Q.1 Define meristem. Write its characteristic features.

A meristem is a group of young, actively dividing cells found in the growing parts of a plant. It is also called meristematic tissue. Its cells keep dividing by mitosis, so the meristem is mainly responsible for the growth of the plant.

Characteristic features

Cells are young, undifferentiated, small, living and thin walled.
Cells are isodiametric (oval or round) and compactly arranged, so there are no intercellular spaces.
Each cell has a big, clear nucleus and dense cytoplasm.
Vacuoles are generally absent, and reserve food and other cell inclusions are absent.
Cells show a high rate of metabolism.
Cells keep the power of division (mitosis).

Q.2 Classify meristem on the basis of the location and explain each with example.

On the basis of location, meristems are of three types.

1. Apical meristem. It is located at the tip (apex) of plant parts such as root, stem and floral bud. It increases the length or height of the plant. Example: root apex and shoot apex.
2. Lateral meristem. It lies along the circumference (side) of the plant part. Its activity increases the thickness (girth) of the plant. Example: vascular cambium in the stem of dicots.
3. Intercalary meristem. It lies at the lower part of internodes and at the bases of leaves. It is common in monocots and is cut off from the apical meristem. It increases the length of the internode and helps lodged cereals and grasses to stand erect again. Example: base of internodes of maize and sugarcane. It is absent in rosette plants such as cabbage.

Q.3 What is parenchyma? Discuss its modifications.
Class 12 Biology Unit 1 Plant Anatomy notes - different types of parenchyma

Figure: Different types of parenchyma

Parenchyma is the most common simple permanent tissue. It is a group of living cells that are oval or spherical, with thin cellulose walls, a nucleus, cytoplasm and vacuole. The cells are loosely arranged with intercellular spaces. It is found in the soft parts of the plant and mainly stores food.

Modifications of parenchyma

1. Epiblema / epidermis. A single outermost layer of root, stem and leaf. It protects the plant, and in roots it helps in the absorption of water and minerals.
2. Chlorenchyma. Parenchyma with chloroplasts. It is present in the mesophyll of leaves and in the outer cortex of young stems, and it carries out photosynthesis.
3. Aerenchyma. Parenchyma with large air cavities. It is common in aquatic floating plants and makes them light and buoyant.
4. Prosenchyma. Elongated, thick-walled, compactly arranged living cells, found in the pericycle and conjunctive tissue. It gives extra support.
5. Idioblast. Large parenchyma cells that store cell inclusions such as tannins, oils and crystals.

Q.4 Compare between fibre and sclereid.
Class 12 Biology Unit 1 Plant Anatomy notes - sclerenchyma fibre and sclereid

Figure: Sclerenchyma, a fibre and a sclereid

FibreSclereid
Much elongated, narrow, spindle-shaped cell.Isodiametric (nearly round) cell, also called stone cell.
Ends are tapering (pointed).Cell is short and may be branched.
Walls are thick and lignified, lumen is narrow.Walls are thicker, with a higher degree of lignification.
Of three types: surface fibres, wood fibres and bast fibres.Makes the protective layer of seeds and hard fruits.
Gives mechanical support. Fibres of jute, hemp and flax are used to make commercial products.Gives hardness. Grittiness of guava and pear is due to sclereids, and the stony shell of coconut and walnut is made of them.

Q.5 Define complex permanent tissue and write its types.

A complex permanent tissue is a group of different kinds of cells that work together as one unit to do a specific function. The cells differ in origin, shape, size and nature. Because they mainly conduct materials, they are also called conducting tissues or vascular tissues.

Types:

Xylem (wood or hadrome): conducts water and minerals upwards from the root. It has tracheids, vessels, xylem parenchyma and xylem fibres.
Phloem (bast or leptome): conducts prepared organic food in both directions. It has sieve tubes, companion cells, phloem parenchyma and phloem fibres.
Q.6 Differentiate between xylem and phloem.
XylemPhloem
Also called wood or hadrome.Also called bast or leptome.
Conducts water and minerals.Conducts prepared organic food.
Flow is one way, from root to other parts (upward).Flow is in both directions.
Made of tracheids, vessels, xylem parenchyma and xylem fibres.Made of sieve tubes, companion cells, phloem parenchyma and phloem fibres.
Mostly dead cells (only xylem parenchyma is living).All cells are living except phloem fibres.
Gives mechanical strength.Does not give much mechanical strength.

Q.7 Study the given figure and answer the following question.
Class 12 Biology Unit 1 Plant Anatomy notes - figure for Q.7

Figure for Q.7

The figure shows a vascular bundle in which phloem (outer side) and xylem (inner side) lie together on the same radius, and there is no strip of cambium between them.

b) Name the type of vascular bundle?

It is a conjoint, collateral and closed vascular bundle. Conjoint, because xylem and phloem are in the same bundle. Collateral, because phloem lies on the outer side of xylem. Closed, because there is no cambium between them.

c) Which type of plants have such vascular bundles in stem?

Monocotyledonous plants (monocots), for example maize. Their vascular bundles are scattered in the ground tissue.

d) Is secondary growth possible in such a vascular bundle? Why?

No. Secondary growth needs a cambium to make new xylem and phloem. This bundle has no cambium, so it cannot show secondary growth.

Q.8 Mention the characteristic features of dicot root anatomy.

A transverse section of a young dicot root shows these features.

Epiblema: the outermost single layer of thin-walled cells, without cuticle and stomata. Some cells form unicellular root hairs that absorb water and minerals.
Cortex: a broad zone of parenchyma with intercellular spaces, without chloroplasts. It stores food and conducts water from the epiblema inwards.
Endodermis: a single layer of barrel-shaped cells. Casparian strips (suberin and lignin) are present on their radial and tangential walls. The thin-walled passage cells opposite the xylem allow water to pass.
Pericycle: one or a few layers of living cells inside the endodermis. It gives rise to lateral roots and to the vascular cambium during secondary growth.
Vascular bundles: radial type, xylem and phloem alternate on different radii. There are only 2 to 6 patches (diarch to hexarch). Xylem is exarch.
Conjunctive tissue: parenchyma between xylem and phloem. It helps in forming the cambium later.
Pith: very small or absent.

Q.9 Compare between the anatomy of dicot root and monocot root.
Dicot rootMonocot root
Vascular bundles are few: 2 to 6 (diarch to hexarch).Vascular bundles are many: more than 6 (polyarch).
Pith is very small or absent.Pith is large and well developed.
Conjunctive tissue is parenchyma and it takes part in forming cambium.Conjunctive tissue is parenchyma and it may become sclerenchyma in old roots.
Pericycle gives rise to lateral roots and vascular cambium.Pericycle gives rise to lateral roots only.
Cambium forms later, so secondary growth takes place.Cambium is absent, so there is no secondary growth.
Cortex is made of parenchyma only.Cortex of old roots may have a sclerenchymatous exodermis.

Q.10 Study the given figure and answer.
Class 12 Biology Unit 1 Plant Anatomy notes - figure for Q.10

Figure for Q.10

The figure shows a transverse section of a stem. It has an epidermis with hairs, a hypodermis, a cortex, an endodermis, a pericycle, vascular bundles in a ring with cambium in each bundle, medullary rays and a central pith.

a) Name the given figure.

Transverse section (T.S.) of a young dicot stem.

b) What is hypodermis made up of?

The hypodermis lies just below the epidermis. It is made of 3 to 4 layers of collenchyma cells, which have no intercellular spaces because of the pectin deposit. It gives mechanical support to the young stem.

c) Write about its vascular bundles.

The vascular bundles are arranged in a ring (eustele). They are conjoint, collateral and open (a strip of cambium is present between xylem and phloem). Phloem lies towards the outside, just below the sclerenchymatous bundle caps of the pericycle, and xylem lies towards the pith. Xylem is endarch, because the protoxylem is towards the pith. The bundles are separated by the medullary rays.

Q.11 Draw the labeled diagram of monocot stem.
Class 12 Biology Unit 1 Plant Anatomy notes - anatomy of monocot stem

Figure: Plant anatomy of a monocot stem, T.S.

A transverse section of a monocot stem is drawn as below. Its main parts are listed under the diagram.

Epidermis: single outer layer with a thick cuticle, no hairs. It has stomata with dumb-bell shaped guard cells.
Hypodermis: a few layers of sclerenchyma cells below the epidermis. It gives mechanical strength.
Ground tissue: parenchyma cells with intercellular spaces. It is not divided into cortex, endodermis, pericycle, pith and medullary rays.
Vascular bundles: many, scattered in the ground tissue (atactostele). They are conjoint, collateral and closed, smaller and more numerous near the periphery, larger and fewer near the centre. Each bundle has a sclerenchymatous bundle sheath.
Xylem: vessels are arranged in a V or Y shape. The two large metaxylem vessels are towards the outside and the protoxylem towards the inside. A water cavity is formed by the protoxylem.
Phloem: sieve tubes and companion cells on the outer side. Phloem parenchyma and cambium are absent.
Q.12 How does dicot leaf differ from monocot leaf? Write your answer based on stomatal distribution, nature of mesophyll tissue, shape of guard cells, bundle sheath extensions.
BasisDicot leafMonocot leaf
Stomatal distributionMany stomata on the lower epidermis, and few or none on the upper epidermis.Stomata are present on both upper and lower epidermis, almost equally.
Nature of mesophyll tissueDifferentiated into palisade layer (upper) and spongy layer (lower).Not differentiated into palisade and spongy layers. It is homogeneous.
Shape of guard cellsKidney (bean) shaped.Dumb-bell shaped.
Bundle sheath extensionMade of collenchyma cells.Made of dead sclerenchyma cells.
Bulliform cellsAbsent.Present in the upper epidermis of some monocots (maize). They help in rolling of the leaf.
Vascular bundlesOf different sizes, arranged in a net (reticulate venation), conjoint, collateral and closed.Of different sizes, parallel to each other (parallel venation), conjoint, collateral and closed.

Q.13 What is periderm? Explain its components with labeled diagram.
Class 12 Biology Unit 1 Plant Anatomy notes - anatomy of periderm

Figure: Anatomy of periderm (phellem, phellogen and phelloderm)

Periderm is the protective tissue formed in the stem and root of dicots and gymnosperms during secondary growth. It replaces the epidermis, which breaks because of the increase in thickness. It is made of three layers, formed by the activity of cork cambium:

Phellogen (cork cambium): a ring of meristematic cells formed in the outer cortex. It divides to form tissues on both sides, more on the outer side.
Phellem (cork): formed on the outer side of phellogen. It has dead, compactly arranged rectangular cells with suberised walls. It is impervious to water and microbes and protects the inner living tissues.
Phelloderm (secondary cortex): formed on the inner side of phellogen. It has living, thin-walled parenchyma cells that store food.

At some places the phellogen forms loosely arranged complementary cells instead of cork. They rupture the epidermis to form lenticels, the open pores for gas exchange.


Q.14 Differentiate between early-wood and late-wood.
Early wood (spring wood)Late wood (autumn wood)
Formed in spring, when the cambium is more active.Formed in dry summer or cold winter, when the cambium is less active.
Vessels are more and have wider cavities.Vessels are fewer and have narrow cavities.
Wood is lighter in colour and less dense.Wood is darker in colour and denser.
The band is broad and forms the major part of the ring.The band is narrow and thin.

Long Answer Questions

Q.1 Define plant tissues. Show the flow chart of different types of plant tissues.

A tissue is a group of cells with a common origin that carry out a specific function. In a plant, tissues build the organs (root, stem, leaf), and the order of organisation is cell, tissue, organ, organ system, organism. Plant tissues can be studied under the microscope by cutting a thin section of the plant part.

Plant tissues are classified on the basis of the power of cell division into meristematic tissue and permanent tissue. The flow chart is shown below.

Plant tissuesMeristematic tissuePermanent tissueApical meristemIntercalary meristemLateral meristemSimple permanent tissueParenchymaCollenchymaSclerenchymaComplex permanent tissueXylemPhloemSpecial (secretory) tissueLaticiferous tissueGlandular tissue

Figure: Plant anatomy flow chart showing the types of plant tissues


Q.2 Describe the types of simple permanent tissues with diagrams.

A simple permanent tissue is made of similar cells that have a common origin and do the same work. It is of three types.

1. Parenchyma

Living, oval or spherical cells with thin cellulose walls, nucleus, cytoplasm and vacuole.
Cells are loosely arranged with intercellular spaces.
Function: storage of food. Its modifications do other work: chlorenchyma (photosynthesis), aerenchyma (buoyancy), prosenchyma (support) and idioblast (storage of inclusions).
Class 12 Biology Unit 1 Plant Anatomy notes - different types of parenchyma

Figure: Different types of parenchyma

2. Collenchyma

Living, elongated or oval cells with unevenly thickened walls because of pectin. There are little or no intercellular spaces.
It is present in the hypodermis of petiole, pedicel and young dicot stems, and forms the bundle sheath extension in dicot leaves.
Types: angular, lacunar and lamellar collenchyma.
Function: gives flexibility and mechanical support. So it is called the living mechanical tissue. It also stops tearing of leaves.
Class 12 Biology Unit 1 Plant Anatomy notes - collenchyma diagram

Figure: Collenchyma (plant anatomy of simple permanent tissue)

3. Sclerenchyma

Dead, elongated, narrow cells without intercellular spaces. The walls are very thick and impermeable because of lignin, and the lumen is narrow.
It gives strength, rigidity and protection to the plant parts. It is present in seed coats, hypodermis of monocot stem, bundle sheath and stony fruits like coconut.
Types: fibres (long, spindle-shaped with tapering ends) and sclereids (stone cells, isodiametric).
Class 12 Biology Unit 1 Plant Anatomy notes - sclerenchyma fibre and sclereid

Figure: Sclerenchyma, a fibre and a sclereid

Q.3 What are vascular bundles? Classify them on the basis of their arrangement. Describe each type with diagram.

A vascular bundle is a strand of the vascular system of the plant. It is made of xylem and phloem, and cambium may or may not be present. It can be seen under the microscope in any plant part during primary growth.

On the basis of arrangement of xylem and phloem, vascular bundles are of three types.

1. Radial type. Xylem and phloem lie at alternate positions on different radii. It is found in roots.

Class 12 Biology Unit 1 Plant Anatomy notes - Radial vascular bundle

Figure: Radial vascular bundle (xylem and phloem on different, alternating radii)

2. Conjoint type. Xylem and phloem are attached together as a single bundle. It is of three types.

Collateral: phloem lies on the outer side of xylem. If a strip of cambium is present between them, it is conjoint, collateral and open (example: sunflower stem). If the cambium is absent, it is conjoint, collateral and closed (example: monocot stem).
Bicollateral: xylem lies between the outer phloem and the inner phloem. Cambium is present on both sides of xylem (example: cucumber stem).
Class 12 Biology Unit 1 Plant Anatomy notes - conjoint vascular bundle

Figure: Conjoint type of vascular bundle (collateral and bicollateral)

3. Concentric type. One of xylem and phloem is completely surrounded by the other. It is of two types.

Amphicribral: xylem is in the centre and is surrounded by a ring of phloem. It is seen in pteridophytes (ferns) and aquatic hydrophytes.
Amphivasal: phloem is in the centre and is surrounded by xylem. It is seen in the stem of Dracaena and Yucca.
Class 12 Biology Unit 1 Plant Anatomy notes - concentric vascular bundle

Figure: Concentric type of vascular bundle (amphicribral and amphivasal)


Q.4 Discuss the root anatomy of a monocot with suitable diagram. Mention any three diagnostic features of it.

The transverse section of a typical monocot root (for example maize) shows the following tissues.

Class 12 Biology Unit 1 Plant Anatomy notes - anatomy of a monocot root

Figure: Plant anatomy of a monocot root, T.S. (labelled)

1. Epiblema: the outermost single layer of compactly arranged parenchyma cells with no intercellular space. It has no stomata and bears unicellular root hairs.
2. Cortex: a multi-layered region of oval parenchyma cells with intercellular spaces, which help in gas exchange and storage of starch. In old roots a few layers below the epiblema form a sclerenchymatous exodermis for support.
3. Endodermis: the innermost layer of the cortex, of barrel-shaped cells forming a ring around the stele. Casparian strips of suberin and lignin are present. Passage cells are usually absent.
4. Pericycle: a single layer of thin-walled cells inside the endodermis. It gives rise to lateral roots only.
5. Vascular bundles: radial, many (polyarch, more than 6). Xylem is exarch. The protoxylem has spiral or annular thickening, and the metaxylem has wide vessels with reticulate or pitted thickening. Phloem has sieve tubes, companion cells and phloem parenchyma, but phloem fibres are usually absent.
6. Conjunctive tissue: parenchyma that separates xylem and phloem. It may become sclerenchymatous in old roots.
7. Pith: large and well developed, of thin-walled parenchyma cells that store a lot of starch.

Three diagnostic features of the monocot root

Vascular bundles are polyarch (more than six xylem and phloem patches).
Pith is large and well developed.
Cambium is absent, so there is no secondary growth.

Q.5 What is secondary growth? Describe the growth of stele with relevant diagram.

Secondary growth is the increase in the thickness (girth) of the root, stem and branches. It is brought about by the lateral meristems, the vascular cambium and the cork cambium. It takes place mainly in dicots and gymnosperms, and not in monocots, pteridophytes and leaves.

Growth of stele (activity of the vascular cambium)

The widening of the stele by the vascular cambium is called intra-stelar secondary growth. It takes place in the following steps.

Class 12 Biology Unit 1 Plant Anatomy notes - growth of stele in secondary growth

Figure: Growth of the stele during secondary growth in a dicot stem

a. Formation of cambium ring. In a young dicot stem, a strip of intra-fascicular cambium lies between xylem and phloem of each bundle. At the start of secondary growth, some cells of the medullary rays between the bundles become meristematic and form the inter-fascicular cambium. Both join to make a complete cambium ring. It has fusiform initials (elongated) and ray initials (short).
b. Formation of secondary vascular tissues. The fusiform initials divide. The cells cut off towards the inner side form secondary xylem, and those towards the outer side form secondary phloem. More cells are cut off inwards, so a large amount of wood forms and the cambium ring moves outwards. The primary xylem and primary phloem become far apart.
c. Formation of secondary medullary rays. The ray initials form narrow bands of living parenchyma through the secondary xylem and phloem. They conduct food, water and minerals in the radial direction.
d. Formation of annual rings. In temperate climate the cambium is more active in spring and forms wide-cavity vessels (spring or early wood). In winter or dry summer it is less active and forms narrow vessels (autumn or late wood). One spring wood and one autumn wood make one annual ring. The age of the tree can be found by counting the rings (dendrochronology).
e. Formation of heartwood and sapwood. In old trees the inner secondary xylem loses its conducting power. Tyloses, tannins, resins and gums fill the lumen, so the wood becomes dark, hard and durable. This is heartwood (duramen). It gives only mechanical support. The outer younger xylem is lighter in colour, conducts water and stores food. This is sapwood (alburnum).

Extra-stelar secondary growth. The increase in girth ruptures the epidermis and cortex. The outer cortical cells form a cork cambium (phellogen), which makes the periderm (phellem, phellogen and phelloderm) and lenticels. So secondary growth in a dicot stem forms wood in the centre and periderm at the periphery.

Significance: it gives timber, makes the stem strong enough for the tree to grow tall, gives the age of the tree through annual rings, and the corky bark protects the inner tissues.

4. Plant Anatomy Notes PDF

PDF Notes 1

PDF Notes 2

5. Plant Anatomy: Frequently Asked Questions

What is plant anatomy? (Plant anatomy notes)
Plant anatomy is the study of the internal structure of plants, mainly the tissues of root, stem and leaf, seen by cutting thin sections and observing them under a microscope. Nehemiah Grew (1641-1712) is known as the father of plant anatomy. Plant anatomy and physiology are closely related, because the structure of a tissue decides its function.
What are the types of plant tissues?
On the basis of the power of cell division, plant tissues are meristematic tissue (apical, intercalary and lateral meristem) and permanent tissue. Permanent tissue is simple (parenchyma, collenchyma, sclerenchyma), complex (xylem and phloem) or special (laticiferous and glandular tissue).
What is the difference between xylem and phloem?
Xylem conducts water and minerals upwards and is mostly dead, with tracheids, vessels, xylem parenchyma and xylem fibres. Phloem conducts prepared food in both directions and is living except the phloem fibres, with sieve tubes, companion cells, phloem parenchyma and phloem fibres.
What are the types of vascular bundles?
Vascular bundles are radial (xylem and phloem on different radii, as in roots), conjoint (collateral open or closed, and bicollateral) and concentric (amphicribral and amphivasal). Dicot stem has conjoint, collateral and open bundles, and monocot stem has conjoint, collateral and closed bundles.
What is the difference between dicot and monocot root, stem and leaf?
A dicot root has 2 to 6 xylem patches, small pith and secondary growth, while a monocot root is polyarch with a large pith and no secondary growth. A dicot stem has bundles in a ring with cambium, and a monocot stem has scattered closed bundles in a ground tissue. A dicot leaf has palisade and spongy mesophyll and kidney-shaped guard cells, and a monocot leaf has undifferentiated mesophyll and dumb-bell shaped guard cells.
What is secondary growth in a dicot stem?
Secondary growth is the increase in girth of the stem by the vascular cambium and the cork cambium. The vascular cambium forms secondary xylem inside and secondary phloem outside, giving annual rings, heartwood and sapwood. The cork cambium forms the periderm, made of phellem, phellogen and phelloderm.
Where can I get plant anatomy pdf, plant anatomy notes pdf, plant anatomy ppt and plant anatomy book?
This page has two plant anatomy notes PDF files that you can read online in the viewers of the PDF section, along with all the solved exercise questions of the chapter and the plant anatomy diagram for each answer. For a plant anatomy book, use your Class 12 Biology textbook, and use these notes to revise. For a plant anatomy ppt, the diagrams and flow chart of this page can be copied into your own slides for study.
Where can I get Class 12 Biology Book PDF Nepal and Class 12 Biology Book PDF Asmita Publication?
The Class 12 Biology book of Asmita Publication is a textbook of its publisher, and this page does not claim to host it. These notes follow the NEB Class 12 Biology syllabus of Unit 1 Plant Anatomy, solve the exercise questions of the chapter and add the missing diagrams, so you can use them together with the textbook.
Where can I get Class 12 biology notes, Class 12 biology pdf and Class 12 biology notes pdf for chapter 1?
Class 12 biology chapter 1 is Plant Anatomy, and this page has its notes, the solved multiple choice questions and the short and long answer questions, with two PDF notes. The other chapters of Class 12 Biology are on the Class 12 Biology page of the website.
Where can I get Class 12 Biology notes NEB (Nepal) in the style of Note Mandir?
The Class 12 Biology notes of the NEB syllabus are given on this website chapter by chapter. Students who look for notes in the style of Note Mandir will find this chapter in the same way: syllabus, micro syllabus, solved exercise with diagrams, and PDF notes. These are NEB Class 12 Biology notes for Unit 1 Plant Anatomy.
What topics are included in Unit 1 Plant Anatomy of NEB Class 12 Biology?
The unit covers the concept of tissue, the types of plant tissues (meristems and permanent tissues), the anatomy of dicot and monocot root, stem and leaf, and the secondary growth of the dicot stem.

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